Enzyme Catalase Labs

Varibles that excise Enzyme Catalysis Reaction ranges admission Molecules ar continuously moving in our bodies and in nature. When molecules move fast generous they jolt into whizz another, entirelyowing chemic substance responses to eliminate. Factors such(prenominal) as temperature and closenesss peck either answer increase or pass these chemical receptions. (Jubenville. ) Enzymes atomic number 18 known as catalyst be hit they are subject to fixity up chemical reaction ordinates without organism destroyed or altered. They are equal to(p) to encourage chemical reactions by lessen the energy of activation.The chief(prenominal) parting of enzyme catalase is to convert henry total heat bl individually ( weewee2) in our bodies into type O and water. This screwing be visually seen when henry total heat peroxide is assign on a wound and the peroxide bubbles. Enzymes set up in like manner be free-base in plant cells and fungi. (Huston. ) In this essay we sort the many variables that can transmit the calculate of this reaction such as temperature, tightfistedness force back aims of enzyme catalase and pH values. We are able to track these changes using an O2 be adrift sensor. (Enzymes. ) It is predicted that the charge per unit of reaction will increase with temperature, pH levels and concentration. MethodsThree running tubings were each alter with 5 mL of 3% hydrogen peroxide and 5 mL of water. 10 drops of enzymes pause system was consequently added to the Naigene chamber for each observation. trial furnishs oneness, two and three were added to the Naigene chamber respectively. The O2 turgidness Sensor was situated on superlative degree of the Naigene chamber. The Naigene chamber was swirled for 60 seconds while the O2 grease-gun Sensor save the type O macrocosm released during the reaction. The results were preserve. To study the military groups of enzyme concentration on rate of reaction, cardinal a nalyse tubes were each alter with 5 mL of 3% hydrogen peroxide and 5 mL of water.For each scrutiny observation 5, 10, 15 and 20 drops of enzyme catalase were fixed in the Naigene chamber. The four test tubes were then added respectively. The Naigene chamber was swirled for 60 seconds while the O2 gasoline Sensor recorded the oxygen macrocosm released during the reaction. To test the effect of temperature on reaction rate, three test tubes were each fill with 5 mL of 3% hydrogen peroxide and 5 mL of water. For each observation 10 drops of enzyme catalase was added to the Naigene chamber. assay tube one was staind in ice (temperature of 0-5 C). try out tube wo was placed in room temperature (20-25 C). probe tube three was placed in warm water (30-35 C). Each test tube was held in this environment for v minutes. The Naigene chamber was swirled for 60 seconds while the O2 Gas Sensor recorded the oxygen creation released during the reaction. To measure the effect of pH on ca talase activity, three test tubes were each filled with 5 mL of 3% hydrogen peroxide and 5 mL of the enamor pH buffer. streamlet tube one was filled with 5 mL of pH 4. trial tube two was filled with 5 mL of pH 7. Test tube three was filled with 5 mL of pH 10.Ten drops of enzyme catalase was added to the Naigene chamber and test tube one, two and three were added respectively. The O2 Gas Sensor was placed on hint of the Naigene chamber and was swirled for 60 seconds. The O2 Gas Sensor then recorded the oxygen being released during the reaction. To measure the effect of different substrare concentrations on catalase reactions, three test tubes were use and designate one, two and three. Test tube one was filled with 3 mL of 3% hydrogen peroxide and 7 mL of water. Test tube two was filled with 5 mL of 3% hydrogen peroxide and 5 mL of water.Test tube three was filled with 7 mL of 3% hydrogen peroxide and 3 mL of water. 10 drops of catalase suspension was placed in the Naigene bottle for each observation. Test tube one, two and three were then added to the Naigene chamber respectively. The O2 Gas Sensor was placed on top of the Naigene chamber and was swirled for 60 seconds. The O2 Gas Sensor then recorded the oxygen being released during the reaction. Results number 1 Test pipe Number stray of sign Reaction (m) 1 0. 085282 2 0. 074574 3 0. 09223 think 1 The amount reaction rate of the enzyme concentration. foresee 2 Test Tube Drops of enzyme suspension rank of Initial Reaction (m) 1 5 0. 060459 2 10 0. 071033 3 15 0. 0966 4 20 0. 15003 Figure 2 Changes in reaction rate due to the enzyme concentration. Figure 3 Test Tube Temperature metric Rate of Initial Reaction (m) 1 0-5 C 0. 038694 2 20-25 C 0. 084487 3 30-35 C 0. 065194 Figure 3 Changes in reaction rate due to the make of different temperatures. Figure 4 Test Tube pH level Rate of Initial Reaction (m) 1 4 0. 013519 2 7 0. 045141 3 10 0. 049314Figure 4 Changes in reaction rate due to the pH level of the solution. Figure 5 Test Tube Amount of H2O2 Amount of H2O Rate of Initial Reaction (m) 1 3 7 0. 027672 2 5 5 0. 09168 3 7 3 0. 1087 Figure 5 Changes in reaction rate due to different ratios of 3% hydrogen peroxide (H2O2) and water (H2O) In mannikin 1, we can see that the figures for each test were relatively the same. This is because the amount and type of chemicals used in each test were the same. Figure two shows the initial rate of reaction increasing as the amount of enzyme suspension increases.This evidence demonst grade that the enzyme suspension allowed the reaction to occur to a greater extent rapidly. Figure 3 demonstrates how temperature can play a role in rate of reaction. Our figures show that showed that rate of reaction was at a peak when in medium temperatures. Various levels of pH also played a role in rate of reaction. Figure 4 demonstrates that the high the pH level, the faster reaction rate was. Figure 5 demonstrates that different ratios of H2O2 and H2O ca n alter the rate of reaction. The high amounts of H2O2 allowed higher reaction rates then the lower concentrated amounts.Discussion Enzymes are accountable for almost all chemical reactions that take place. They are made up of proteins and are considered biocatalysts. (Jubenville. ) Biocatalysts can be described as when enzymes are used as catalysts to cause chemical reactions. (Novasep. ) Enzymes are known as catalyst because they are able to speed up reaction rates without being destroyed or altered. They are able to encourage chemical reactions by decreasing the energy of activation. (Huston. ) Enzymes pull back substrates to their surface allowing chemical reactions to occur.Every enzyme haves reactive web positions which allow very special chemical reactions. The shape of the reactive site on the enzyme and the shape of the reactive site on the substrate must all told match in order for them to attract to one another. (Jubenville. ) Enzyme catalase can be raise in vario us places of our bodies and nature. The main function of enzyme catalase is to convert hydrogen peroxide (H2O2) in our bodies into oxygen and water. This can be visually seen when hydrogen peroxide is put on a wound and the peroxide bubbles. (Huston. ) It can also be found in nature in plants and fungi.These molecules are constantly moving. When moving fast enough they collide into one another, allowing chemical reactions to occur. Factors such as temperature and concentrations can either help decrease or increase these reactions. Concentration of enzyme catalase for example, plays a huge role of how much oxygen will be broken down. Concentrations of enzyme catalase can also increase obtains of a chemical reaction occurring because there are more molecules unattached to do the job. The higher concentration of enzyme catalase used, the more oxygen will be released during reaction.The temperatures of the environment in which these reactions take place also play a essential role on t he reaction. Heat for example, speeds up the movement of molecules allowing more of a chance for them to collide and cause a chemical reaction. (Jubenville. ) pH factors also change reaction rates. pH stands for power of hydrogen and measures the concentration on hydrogen ions in a solution. (Hyperphysics. ) The higher the concentration, the more hydrogen ions available to be broken down by enzymes. The more hydrogen or hydrogen eroxide in a solution, the more oxygen being released during the reaction. It was expected that reaction rates would increase with higher concentrations of H2O2, pH levels, temperatures and ratios. This was all proven true through our observations of our experiment.Works Cited Biocatalysis comment of Biocatalysis in Novasep Glossary. Biocatalysis Definition of Biocatalysis in Novasep Glossary. Novasep, 2010. Web. 1 Oct. 2012. <http//www. novasep. com/misc/glossary. asp? defId=49>. (Novasep. ) Enzymes. Enzymes. Tuberose, n. d. Web. 27 Sept. 2012. <ht tp//www. uberose. com/Enzymes. hypertext mark-up language>. (Enzymes. ) Frequently Asked Questions A Learn A Houston Enzymes. Frequently Asked Questions A Learn A Houston Enzymes. Huston Enzymes, 2010. Web. 1 Oct. 2012. <http//www. houston-enzymes. com/learn/faq. php>. (Huston. ) Jubenville, Robert B. , and Richard G. Thomas. General biology Laboratory Manual. Third ed. Dubuque Kendall/Hunt, 2008. Print. (Jubenville. ) PH. As a Measure of Acid and Base Properties. Hyperphysics, n. d. Web. 5 Oct. 2012. <http//hyperphysics. phy-astr. gsu. edu/hbase/chemical/ph. html>. (Hyperphysics. )